Asymmetrical Flow Field-Flow Fractionation (AF4) and Centrifugal Field-Flow Fractionation (CF3) are high resolution separation techniques for fractionation of macromolecules and biological nanoparticles based on their hydrodynamic size (AF4) and mass (CF3).
Exosomes are small extracellular vesicles containing nucleic acid and proteins that show great promise for use in cancer diagnostics and therapeutic applications. As characterization of exosomes had proven challenging with traditional separation techniques due to their inherent heterogeneity and complexity, the Utah researchers turned to a multiple fractionation approach to solve their application challenge.