CRISPR technology has greatly facilitated gene editing. Associate Professor Thorsten Müller from Ruhr-Universität Bochum and Dr. Hassan Bukhari from Harvard Medical School discuss its pros and cons in a review article in the journal “Trends in Cell Biology” from 12 September 2019. They believe Crispr technology has future potential primarily if it can be rendered usable in the field of stem cell research.
In order to analyze the effects of genes or gene products, they used to be artificially over-activated.